Inhalable nanocatchers against SARS-CoV-2
[ABSTRACT]
The recently emerged SARS-CoV-2 variants are more transmissible, which brings new challenges to vaccine treatment. There is an urgent global need for alternative strategies that could effectively and rapidly prevent the infection of various SARS-CoV-2 variants.
Herein, we design human angiotensin-converting enzyme II (hACE2)–containing nanocatchers (NCs) derived from genetically engineered cells stably expressing hACE2 as the competitor with host cells for virus binding, to protect cells from SARS-CoV-2 infection.
An inhalable formulation fabricated by nanocatchers and the muco-adhesive excipient hyaluronic acid could significantly prolong the retention of NCs in the lung and exhibits potent pseudovirus inhibition ability in an hACE2-expressing mouse model. Importantly, the inhalable NCs in the lyophilized formulation allow long-term storage, facilitating their future clinical use.
